Development of a new nano arginase HPLC capillary column for the fast screening of arginase inhibitors and evaluation of their binding affinity

A simple and rapid Nano LC method has been developed for the screening of arginase inhibitors. The is based on immobilization biotinylated a neutravidin functionalized nano HPLC capillary column. step performed by frontal analysis very fast only takes few minutes. miniaturized column 170 nL (length 5 cm, internal diameter 75 ?m) significantly decreased required amount used enzyme (25 pmol). This was significance importance when working with less available or expensive purified enzyme. Non-selective adsorption organic monolith matrix reduced (<6%) efficient yield high (92%). resultant affinity columns showed excellent repeatability long lifetime. reaction product achieved within 60 s immobilized retained 97% initial activity beyond 90 days. novel approach can thus be evaluation recognition assay induced series inhibitor molecules (caffeic acid phenylamide, chlorogenic acid, piceatannol, nor-NOHA acetate) plant extracts.